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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
14:06

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Preparative scale separation of functionally distinct murine B cell subpopulations

R Chaby, R Girard

    Journal of Immunological Methods
    |June 25, 1985
    PubMed

    Mouse splenic B cells were found to be rapidly and reproducibly fractionated by adherence at 4 degrees C to plastic dishes pretreated with an alkaline buffer. The cell subsets obtained exhibited marked differences in size, surface immunoglobulins (sIg), and responses to B mitogens. Adherent B lymphocytes, bearing high densities of sIg, responded particularly to LPS whereas non-adherent B lymphocytes, with low densities of sIg, responded especially to dextran sulfate. The correlations between the separated populations and subsets at distinct maturation stages are discussed.

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