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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
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Sites under positive selection modulate the RNA silencing suppressor activity of rice yellow mottle virus movement

Drissa Sérémé1, Séverine Lacombe2, Moumouni Konaté1

  • 1Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, 01 BP 476 Ouagadougou 01, Burkina Faso.

The Journal of General Virology
|October 5, 2013
PubMed

Insights

The P1 protein of rice yellow mottle virus (RYMV) gene shows adaptive evolution. Mutations in this RNA silencing suppressor gene significantly altered its activity, highlighting evolutionary pressures.

Area of Science:

  • Molecular Biology
  • Virology
  • Evolutionary Biology

Background:

  • RNA silencing is a crucial gene regulation mechanism in eukaryotes, vital for defense against viruses.
  • The P1 protein of rice yellow mottle virus (RYMV) acts as a movement protein and suppresses RNA silencing, with varying efficiency across isolates.

Purpose of the Study:

  • To investigate the adaptive evolution of the RYMV P1 protein gene.
  • To identify sites under positive selection and assess their impact on RNA silencing suppression activity.

Main Methods:

  • Performed a site-by-site analysis of the dN/dS ratio to detect positive selection.
  • Identified 18 positively selected sites in the P1 protein gene.
  • Mutated four identified sites and evaluated their RNA silencing suppression activity in transient expression assays.

Main Results:

  • Identified 18 sites under positive selection within the RYMV P1 protein gene.
  • Mutations at four selected sites quantitatively affected RNA silencing suppression.
  • One mutation significantly decreased suppression, while three mutations significantly increased it.

Conclusions:

  • The RYMV P1 RNA silencing suppressor gene is under adaptive evolution.
  • Specific sites within the P1 protein are critical for its RNA silencing suppression activity and are targets of evolutionary selection.

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