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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
TRIM28 is essential for erythroblast differentiation in the mouse
Tomonori Hosoya1, Mary Clifford, Régine Losson
1Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI;
Blood
|October 5, 2013
Summary
Tripartite motif-containing 28 (TRIM28) is essential for red blood cell development. Its loss in adult mice causes anemia by impairing immature erythroblast maturation in bone marrow.
Area of Science:
- Hematology
- Molecular Biology
- Gene Regulation
Background:
- Tripartite motif-containing 28 (TRIM28) is a transcriptional corepressor involved in various cellular functions.
- TRIM28 has been identified as a cofactor for the NR2C1/NR2C2 nuclear receptor heterodimer, implicated in β-type globin gene repression.
Purpose of the Study:
- To investigate the role of TRIM28 in globin gene regulation and erythropoiesis.
- To determine the necessity of TRIM28 for red blood cell development in vivo.
Main Methods:
- Utilized a conditional loss-of-function in vivo mouse model to study TRIM28.
- Inducibly deleted Trim28 specifically in hematopoietic cells to assess cell-autonomous effects.
Main Results:
- Genetic loss of Trim28 in adult mice resulted in defective immature erythropoiesis and anemia.
- TRIM28 was found to control erythropoiesis in a cell-autonomous manner.
- Absence of TRIM28 led to increased apoptosis and reduced expression of erythroid transcription factors and heme biosynthetic enzymes in immature erythroid cells.
Conclusions:
- TRIM28 is crucial for the cell-autonomous development of immature erythroblasts in the bone marrow.
- TRIM28 plays an essential role in maintaining erythropoiesis and preventing anemia.
