Related Experiment Video
Updated: May 7, 2026

A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice
Published on: November 15, 2024
Antinociceptive activity of Ligusticum porteri preparations and compounds
Krutzkaya Juárez-Reyes1, Guadalupe E Angeles-López, Isabel Rivero-Cruz
1Facultad de Química and.
Context:
The roots and rhizomes of Ligusticum porteri Coulter & Rose (Apiaceae) are widely used in Mexican folk medicine for several purposes, including painful complaints.
Objective:
The main goal of this work was to demonstrate the analgesic action in mice of some preparations and major compounds from L. porteri.
Materials And Methods:
The extracts, aqueous (AE) and organic (OE), the essential oil (EO) and major compounds (10-316 mg/kg) from L. porteri were evaluated as potential antinociceptive agents using the acetic acid-induced writhing and hot plate tests in ICR mice.
Results:
All preparations tested exhibited significant antinociceptive effect in the two animal pain models selected. AE and EO were more effective in the writhing test while OE had a better effect in the hot-plate model. On the other hand, Z-ligustilide (1) provoked an increment in the latency period to the thermal stimuli in the hot-plate test at a dose of 31.6 mg/kg, and a decrease in the number of abdominal writhes at 10 mg/kg. Z-3-butylidenephthalide (2) induced a dose-dependent antinociceptive action in the hot-plate assay; this compound was also effective for controlling the pain provoked by chemical irritation at the doses of 10 and 31.6 mg/kg. Finally, diligustilide (3) inhibited the number of writhing responses at all doses tested but was inactive in the hot-plate model.
Conclusion:
The present investigation provides in vivo evidence supporting the use of L. porteri to treat painful conditions in folk medicine.
Related Concept Videos
Nociception
Analgesia and Pain Management
Ligand-gated Ion Channels
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
Cholinergic Antagonists: Chemistry and Structure-Activity Relationship
Local Anesthetics: Chemistry and Structure-Activity Relationship
Ligand-Gated Ion Channel Receptor: Gating Mechanism