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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrum: Interpretation01:24

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Related Experiment Video

Updated: May 7, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

Multiorder correction algorithms to remove image distortions from mass spectrometry imaging data sets.

Florian Gerber1, Florian Marty, Gert B Eijkel

  • 1Institute of Mathematics, University of Zurich , Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.

Analytical Chemistry
|October 8, 2013
PubMed
Summary

This study introduces two algorithms to correct repetitive distortions in time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging. These methods enhance image quality and analytical usefulness for biological tissue analysis.

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
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Last Updated: May 7, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Materials Science

Background:

  • Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging is crucial for mapping small molecule distributions in biological tissues.
  • Sequential image acquisition in TOF-SIMS can introduce repetitive, tile-to-tile distortions, compromising data quality and analytical utility.
  • Existing methods may not adequately address these specific artifact types.

Purpose of the Study:

  • To statistically characterize repetitive distortions in TOF-SIMS imaging data.
  • To develop and validate novel algorithms for correcting these specific artifacts.
  • To assess the impact of correction methods on image quality and spectral data integrity.

Main Methods:

  • Statistical analysis of repetitive artifacts in TOF-SIMS image tiles.
  • Development of two correction algorithms: a generalized linear model and linear discriminant analysis.
  • Simulation studies using repetitive and non-repetitive tiling errors to evaluate algorithm performance.

Main Results:

  • Both developed algorithms effectively correct repetitive distortions in TOF-SIMS images.
  • Image quality is significantly improved for both positive and negative ion modes.
  • Correction methods do not alter the spectral component of the dataset, preserving chemical information.

Conclusions:

  • The proposed algorithms substantially enhance the analytical usefulness of distorted TOF-SIMS imaging data.
  • These methods provide a robust solution for addressing common artifacts in TOF-SIMS imaging of biological tissues.
  • The spectral integrity is maintained, ensuring reliable molecular distribution analysis.