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Related Experiment Video

Updated: May 7, 2026

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
06:09

Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

Probiotic modulation of dendritic cell function is influenced by ageing.

Jialu You1, Honglin Dong1, Elizabeth R Mann2

  • 1Department of Food and Nutritional Sciences, The University of Reading, Reading, UK.

Immunobiology
|October 8, 2013
PubMed
Summary

Probiotics enhance dendritic cell (DC) function in older adults, particularly cytokine production. However, this improvement does not fully restore T-cell responses in aged individuals, indicating the persistent effects of immunosenescence.

Keywords:
AgeingAllogeneic mixed leucocyte reactionCFSECytokineDCsDendritic cellsL. casei ShirotaLcSMLRPAMPsPRPsProbioticscarboxyfluorescein diacetate succinimidyl esterdendritic cellsmixed leucocyte reactionpathogen recognition patternspathogen-associated molecular patterns

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Area of Science:

  • Immunology
  • Microbiology
  • Gerontology

Background:

  • Dendritic cells (DCs) are crucial for T-cell responses.
  • Probiotics offer health benefits, especially for immunocompromised individuals like the elderly.
  • Age-related decline in immune function (immunosenescence) impacts DC activity.

Purpose of the Study:

  • To investigate the effects of four specific probiotics on DC function in young and older adults.
  • To assess probiotic modulation of DC markers and cytokine production.
  • To evaluate probiotic-enhanced DC interactions with T-cells in an allogeneic mixed leucocyte reaction (MLR) model.

Main Methods:

  • Utilized an allogeneic mixed leucocyte reaction (MLR) model with DCs and T-cells from young and older donors.
  • Tested four probiotics: Bifidobacterium longum bv. infantis CCUG 52486, B. longum SP 07/3, Lactobacillus rhamnosus GG (L.GG), and L. casei Shirota (LcS).
  • Analyzed DC surface marker expression (CD40, CD80, CCR7, CD25) and cytokine production (TGF-β, TNF-α, IL-12, IFNγ, IL-10).

Main Results:

  • All tested probiotics upregulated CD40, CD80, and CCR7 on DCs from both age groups.
  • Probiotics significantly enhanced cytokine production (TGF-β, TNF-α) in DCs from older donors only.
  • Probiotic-pretreated older DCs showed increased activation (CD25) and enhanced stimulation of young T-cells, but did not improve T-cell proliferation in older donors.

Conclusions:

  • Ageing enhances DC responsiveness to probiotics, particularly in cytokine production.
  • Probiotic intervention improves certain DC functions in older adults but does not fully counteract age-related T-cell dysfunction.
  • The study highlights the complex interplay between probiotics, DCs, and immunosenescence.