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Determination of vitamin D and its metabolites

Christopher-John Farrell1, Markus Herrmann

  • 1Laverty Pathology, 60 Waterloo Rd, North Ryde, NSW 2113, Australia.

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Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin D3(cholecalciferol).
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Determination of Michaelis Constant and Maximum Elimination Rate

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Determination of Multiple Dosing Parameters: Loading and Maintenance Doses

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Protein-Drug Binding: Determination Methods

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