Related Experiment Video
Updated: Jan 6, 2026
Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
Published on: February 28, 2025
C-type lectin receptors mRNA expression in patients with otitis media with effusion
Jong Ha Lee1, Dong Choon Park, In Whan Oh
1Physical Medicine and Rehabilitation, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Objective:
The role of C-type lectin receptor, a type of pattern recognition receptor, in otitis media with effusion (OME) is unclear. We assayed the levels of expression of C-type lectin receptor mRNA in children with OME and evaluated its relationship to the presence of bacteria, accompanying diseases, and characteristics of exudates.
Subjects And Methods:
The study population consisted of 73 children with OME who had undergone ventilating tube insertion. The levels of expression of Dectin-1, MR1, MR2, DC-SIGN, Syk, Card-9, Bcl-10, Malt-1, Src, Dec-205, Galectin-1, Tim-3, Trem-1, and DAP-12 mRNA in middle ear effusion were determined by real-time PCR. The level of expression of each mRNA was correlated with the presence of bacteria, accompanying diseases, and exudates characteristics.
Results:
The levels of expression of C-type lectin receptor mRNAs were not associated with bacterial presence or exudates characteristics (p>0.05 each). Levels of expression, however, were significantly higher in patients with sinusitis, adenoid vegetation or adenoiditis, and allergic rhinitis (p<0.05 each).
Conclusions:
Levels of expression of C-type lectin receptor mRNA may be associated with the pathogenesis of OME, being significantly higher in patients with than without accompanying sinusitis, adenoid vegetation or adenoiditis, and allergic rhinitis.
Insights
C-type lectin receptor mRNA levels were higher in children with otitis media with effusion (OME) and accompanying conditions like sinusitis or allergic rhinitis, suggesting a role in OME pathogenesis.
Area of Science:
- Immunology
- Otolaryngology
- Molecular Biology
Background:
- Otitis media with effusion (OME) is common in children, but the role of pattern recognition receptors like C-type lectin receptors (CLRs) is not fully understood.
- Investigating CLRs in OME may reveal new insights into its pathogenesis.
Purpose of the Study:
- To examine the expression levels of various CLR mRNAs in middle ear effusion from children with OME.
- To determine if CLR mRNA expression correlates with bacterial presence, co-existing diseases, or exudate characteristics in OME patients.
Main Methods:
- Real-time PCR was used to quantify mRNA levels of 14 different CLRs and associated signaling molecules (Syk, Card-9, Bcl-10, Malt-1, Src, DAP-12) in middle ear effusion from 73 children with OME.
- Statistical analysis was performed to correlate CLR mRNA levels with clinical data.
Main Results:
- CLR mRNA expression levels did not significantly correlate with the presence of bacteria or the characteristics of middle ear exudates.
- Significantly higher CLR mRNA expression was observed in OME patients with co-existing sinusitis, adenoid vegetation/adenoiditis, or allergic rhinitis.
Conclusions:
- CLR mRNA expression appears to be associated with the pathogenesis of OME, particularly when accompanied by conditions such as sinusitis, adenoid issues, or allergic rhinitis.
- These findings suggest CLRs may play a role in the inflammatory processes underlying OME in specific clinical contexts.
Related Concept Videos
01:15Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
02:23Master Transcription Regulators