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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Budding yeast escape commitment to the phosphate starvation program using gene expression noise.

Noam Vardi1, Sagi Levy, Michael Assaf

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.

Current Biology : CB
|October 8, 2013
PubMed
Summary
This summary is machine-generated.

Budding yeast cells can maintain phosphate starvation responses for many generations after returning to high phosphate conditions. This "commitment" involves feedback loops that reduce internal phosphate levels.

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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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Related Experiment Videos

Last Updated: May 7, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

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Published on: August 10, 2017

Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
17:01

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The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

Area of Science:

  • Cellular biology
  • Molecular genetics
  • Yeast research

Background:

  • Cells need to adapt to nutrient fluctuations, like phosphate availability.
  • In budding yeast, phosphate limitation rapidly triggers the Pho regulon gene expression.
  • This starvation response is mediated by the transcription factor Pho4, which quickly enters the nucleus.

Purpose of the Study:

  • To investigate the long-term regulation of the Pho regulon after phosphate starvation.
  • To understand the mechanisms behind the sustained induction of the Pho regulon.
  • To explore the role of feedback loops and gene expression noise in this sustained response.

Main Methods:

  • Utilized budding yeast models.
  • Investigated Pho4-dependent gene expression.
  • Employed a novel DAmP multiple copy array (DaMCA) method to reduce gene expression noise.

Main Results:

  • The Pho regulon can remain induced for dozens of generations after cells are returned to high phosphate conditions.
  • Approximately 40% of starved cells maintained PHO4-dependent expression for over eleven generations.
  • Two feedback loops, involving PHM1-4 gene induction for phosphate storage and SPL2 induction for reduced uptake, contribute to this sustained activation.
  • Gene expression noise in SPL2 allows for stochastic repression in committed cells.

Conclusions:

  • A 'commitment' mechanism allows yeast cells to maintain the Pho regulon in a high-phosphate environment.
  • This commitment is crucial for preparing cells for future nutrient depletion.
  • Feedback loops and controlled gene expression noise are key components of this adaptive strategy.