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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Updated: May 7, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Published on: March 5, 2019

High-dimensional analysis of human CD8(+) T cell phenotype, function, and antigen specificity.

Evan W Newell1, Wenyu Lin

  • 1Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), Singapore, 138648, Republic of Singapore, evan_newell@immunol.a-star.edu.sg.

Current Topics in Microbiology and Immunology
|October 8, 2013
PubMed
Summary

High-dimensional mass cytometry enhances the identification and characterization of human CD8(+) T cell subsets. This advanced technique provides a comprehensive view of T cells for improved prediction of vaccine efficacy and disease outcomes.

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Last Updated: May 7, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
10:58

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
15:42

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Published on: March 6, 2009

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Antigen-specific T cells are crucial for adaptive immunity.
  • Characterizing diverse T cell subsets is challenging.
  • Human CD8(+) T cells play key roles in health and disease.

Purpose of the Study:

  • To explore the utility of high-dimensional mass cytometry for analyzing antigen-specific T cells.
  • To provide an overview of T cell subsets and their classification.
  • To detail how mass cytometry can characterize T cells based on protein expression, function, and antigen specificity.

Main Methods:

  • Utilized high-dimensional mass cytometry.
  • Analyzed protein expression via surface and intracellular staining.
  • Assessed functional capacity by measuring cytokine production.
  • Determined antigen specificity using peptide-major histocompatibility complex multimers.

Main Results:

  • Mass cytometry offers a more integrated analysis of antigen-specific T cells compared to traditional methods.
  • Enabled concurrent identification and characterization of T cell subsets.
  • Provided a deeper understanding of T cell diversity.

Conclusions:

  • High-dimensional mass cytometry significantly advances the study of antigen-specific T cells.
  • This technique allows for precise measurement of immune responses.
  • Improved prediction of vaccine efficacy and disease outcomes is anticipated.