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Related Concept Videos

Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

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Related Experiment Video

Updated: May 7, 2026

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
07:44

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Published on: February 17, 2023

Tumor suppressor candidate TUSC3 expression during rat testis maturation.

Ahmed Magzoub Khalid1, Atsushi Asano, Yoshinao Z Hosaka

  • 1Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University.

Bioscience, Biotechnology, and Biochemistry
|October 8, 2013
PubMed
Summary

Tumor suppressor candidate 3 (TUSC3) is upregulated in maturing rat testes and prostate. TUSC3 protein is crucial for spermatogenesis, sperm maturation, and prostate development, indicating its role in tumor suppression.

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Teratoma Generation in the Testis Capsule
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Teratoma Generation in the Testis Capsule

Published on: November 7, 2011

Related Experiment Videos

Last Updated: May 7, 2026

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice
07:44

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Published on: February 17, 2023

Teratoma Generation in the Testis Capsule
05:24

Teratoma Generation in the Testis Capsule

Published on: November 7, 2011

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Oncology

Background:

  • Gene expression analysis in developing rat testes identified potential tumor suppressors.
  • Tumor suppressor candidate 3 (TUSC3) emerged as a novel target of interest.

Purpose of the Study:

  • To investigate the role of TUSC3 in spermatogenesis and prostate development.
  • To determine the expression and localization of TUSC3 in normal and developing tissues.

Main Methods:

  • Microarray analysis for gene expression profiling.
  • Reverse-transcription polymerase chain reaction (RT-PCR) and Northern blotting for gene expression validation.
  • Western blotting to detect protein expression.
  • In situ hybridization and immunohistochemistry for mRNA and protein localization.

Main Results:

  • TUSC3 gene expression was significantly upregulated in maturing rat testes and prostate.
  • TUSC3 protein was detected at approximately 40 kDa in testes, prostate, cerebrum, and ovary.
  • mRNA and protein were localized to spermatocytes, Leydig cells in testes, and prostate epithelial cells.

Conclusions:

  • TUSC3 plays a critical role in spermatogenesis, including sperm differentiation and maturation.
  • TUSC3 is involved in normal prostate development.
  • These findings suggest TUSC3 functions as a tumor suppressor in these tissues.