Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary bioactive compounds and inflammaging: Pro-inflammatory triggers and geroprotective countermeasures.

Ageing research reviews·2026
Same author

Natural modulators of enhancer of zeste homolog 2 (EZH2): potential epigenetic regulators of aging-associated pathways.

Biogerontology·2026
Same author

Old worms, new tricks: dynamical instability explains late-life rejuvenation in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Foundations of Gerophysics.

Aging·2026
Same author

Toward actionable interventions in human aging (12th ARDD meeting, 2025).

Aging·2026
Same author

Upskilling in Healthy Longevity Medicine and Its Association With Physicians' Implementation Intent and Self-Reported Clinical Confidence: Cross-Sectional Observational Study.

JMIR medical education·2026

Related Experiment Video

Updated: May 7, 2026

Measurement of Lifespan in Drosophila melanogaster
10:00

Measurement of Lifespan in Drosophila melanogaster

Published on: January 7, 2013

Selective anticancer agents suppress aging in Drosophila.

Anton Danilov1, Mikhail Shaposhnikov, Ekaterina Plyusnina

  • 1Institute of Biology, Komi Science Center, Russian Academy of Sciences, Syktyvkar, 167982, Russia.

Oncotarget
|October 8, 2013
PubMed
Summary

Inhibiting key genes like PI3K, TOR, and NF-κB in fruit flies boosts lifespan and healthspan. Combined rapamycin and wortmannin showed the greatest life extension, improving fly longevity and quality of life.

More Related Videos

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
10:57

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Published on: December 16, 2014

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures
08:55

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Related Experiment Videos

Last Updated: May 7, 2026

Measurement of Lifespan in Drosophila melanogaster
10:00

Measurement of Lifespan in Drosophila melanogaster

Published on: January 7, 2013

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging
10:57

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

Published on: December 16, 2014

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures
08:55

A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures

Published on: November 8, 2024

Area of Science:

  • Gerontology and Molecular Biology
  • Genetics and Gene Regulation
  • Pharmacology and Drug Discovery

Background:

  • Mutations in PI3K, TOR, iNOS, and NF-κB pathways are linked to increased lifespan and reduced age-related diseases in model organisms.
  • Understanding the role of these pathways in aging is crucial for developing interventions.
  • Pharmacological targeting of these pathways offers a potential strategy for promoting longevity.

Purpose of the Study:

  • To investigate the effects of specific inhibitors on the lifespan and quality of life in Drosophila melanogaster.
  • To evaluate the impact of inhibiting PI3K, TOR, iNOS, and NF-κB pathways individually and in combination.
  • To identify potential geroprotective compounds and pathways.

Main Methods:

  • Utilized Drosophila melanogaster as a model organism to study aging.
  • Administered pharmacological inhibitors: wortmannin (PI3K), rapamycin (TOR), 1400W (iNOS), and pyrrolidine dithiocarbamate/QNZ (NF-κB).
  • Assessed lifespan, locomotor activity, and fertility; conducted bioinformatic analysis using KEGG, REACTOME.PATH, DOLite, and GO.BP.

Main Results:

  • Pharmacological inhibition of PI3K, TOR, NF-κB, and iNOS significantly increased Drosophila lifespan without negatively impacting quality of life.
  • The combination of rapamycin (5 μM) and wortmannin (5 μM) yielded the most substantial lifespan extension (23.4%).
  • Bioinformatic analysis corroborated rapamycin's potent aging-suppressor activity, aligning with experimental findings.

Conclusions:

  • Targeting PI3K, TOR, NF-κB, and iNOS pathways pharmacologically is a viable strategy to extend lifespan in model organisms.
  • Combined inhibition, particularly of PI3K and TOR pathways, demonstrates synergistic effects on longevity.
  • Rapamycin emerges as a key compound with significant aging-suppressor properties, warranting further investigation for anti-aging applications.