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A novel non-coding RNA lncRNA-JADE connects DNA damage signalling to histone H4 acetylation

Guohui Wan1, Xiaoxiao Hu, Yunhua Liu

  • 1Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

The EMBO Journal
|October 8, 2013
PubMed

Insights

A novel long non-coding RNA, lncRNA-JADE, is activated by DNA damage and promotes breast tumor growth by enhancing histone acetylation. Its dysregulation may drive tumorigenesis, offering a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Epigenetics

Background:

  • DNA damage response (DDR) is crucial for preventing cancer.
  • Long non-coding RNAs (lncRNAs) are emerging regulators in cellular processes.
  • The role of lncRNAs in DDR and tumorigenesis is under investigation.

Purpose of the Study:

  • To identify novel lncRNAs involved in the DNA damage response.
  • To elucidate the function of lncRNA-JADE in DNA damage and breast cancer.
  • To investigate the mechanism by which lncRNA-JADE influences gene expression.

Main Methods:

  • Identification of lncRNA-JADE via DNA damage induction.
  • Analysis of lncRNA-JADE's dependence on ATM signaling.
  • Assessment of lncRNA-JADE's effect on Jade1 and histone H4 acetylation.
  • Evaluation of lncRNA-JADE expression in human breast tumors.
  • In vivo studies using lncRNA-JADE knockdown in mouse models.

Main Results:

  • A novel lncRNA, lncRNA-JADE, was identified and found to be induced by DNA damage in an ATM-dependent manner.
  • lncRNA-JADE transcriptionally activates Jade1, leading to increased histone H4 acetylation.
  • Elevated lncRNA-JADE levels were detected in human breast tumors compared to normal tissues.
  • Knockdown of lncRNA-JADE significantly suppressed breast tumor growth in vivo.

Conclusions:

  • lncRNA-JADE acts as a critical link between the DNA damage response and histone acetylation.
  • Dysregulation of lncRNA-JADE contributes to breast tumorigenesis.
  • lncRNA-JADE represents a potential diagnostic marker and therapeutic target for breast cancer.

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