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[Gold-silver technic--a new immunohistochemical method for detecting cell membrane antigens]
The gold-silver technique is presented as a new method for immunohistochemical labelling of cell membrane antigens. At first, membrane antigens of mouse lymphocytes, human leukaemic cells, and experimental brain tumour cells are labelled by immunoglobulin- or anti-immunoglobulin-gold complexes. Secondarily, gold colloid particles are made visible in the light microscope by means of subsequent precipitation of metallic silver around the colloid particles according to the principle of photographic development. The gold-silver reaction can be used after direct or indirect gold-labelling of cell membrane antigens. The technique is suitable for both vital and prefixed cells. Labelling of membrane antigens of vital and prefixed cells, however, results in a different pattern of the gold-silver grains. The gold-silver method is easy and quickly to perform, sensitive and not expensive.
The gold-silver technique is presented as a new method for immunohistochemical labelling of cell membrane antigens. At first, membrane antigens of mouse lymphocytes, human leukaemic cells, and experimental brain tumour cells are labelled by immunoglobulin- or anti-immunoglobulin-gold complexes. Secondarily, gold colloid particles are made visible in the light microscope by means of subsequent precipitation of metallic silver around the colloid particles according to the principle of photographic development. The gold-silver reaction can be used after direct or indirect gold-labelling of cell membrane antigens. The technique is suitable for both vital and prefixed cells. Labelling of membrane antigens of vital and prefixed cells, however, results in a different pattern of the gold-silver grains. The gold-silver method is easy and quickly to perform, sensitive and not expensive.