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Updated: May 7, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Characterization of nitrifying microbial community in a submerged membrane bioreactor at short solids retention times
Liang Duan1, Yonghui Song, Siqing Xia
1State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, PR China; Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710, USA.
Abstract:
This study investigated the nitrifying bacterial community in membrane bioreactor (MBR) at short solids retention times (SRTs) of 3, 5 and 10 days. The denaturing gradient gel electrophoresis results showed that different types of ammonia-oxidizing bacteria (AOB) can survive at different operating conditions. The diversity of AOB increased as the SRT increased. The real-time PCR results showed that the amoA gene concentrations were similar when MBRs were stabilized, and it can be a good indicator of stabilized nitrification. The results of clone library indicated that Nitrosomonas was the dominant group of AOB in three reactors. The microarray results showed that Nitrospira was the dominant group of nitrite-oxidizing bacteria (NOB) in the system. All groups of AOB and NOB except Nitrosolobus and Nitrococcus were found in MBR, indicated that the nitrifying bacterial community structure was more complicated. The combination of some molecular tools can provide more information of microbial communities.
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