Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

13.7K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
13.7K
T Cell Types and Functions01:24

T Cell Types and Functions

3.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.2K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

7.1K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.1K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

14.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
14.6K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

65.0K
Overview
65.0K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

2.2K
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Inducible hiPSC-Derived Human Podocyte Model for Functional Analysis of TRPC6 Variants Associated with FSGS.

Cells·2026
Same author

Tyrosinase Cross-Linked PEG Hydrogels with DAT and DATT as Artificial Substrates: Design, Structure, and Functions.

Biomacromolecules·2026
Same author

Perspectives from the 2025 ISCBI/ISCI joint workshop on genetic stability, clonal monitoring, ethical data governance, and global inclusion in stem cell banking.

Stem cell research & therapy·2026
Same author

Development of Stem Cell Data Systems and Associated Data Standards for Cell Therapy.

Advances in experimental medicine and biology·2025
Same author

Promoting the adoption of best practices and standards to enhance quality and reproducibility of stem cell research.

Stem cell reports·2025
Same author

Guidelines for managing and using the digital phenotypes of pluripotent stem cell lines.

Stem cell reports·2024

Related Experiment Video

Updated: May 7, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

52.8K

Culture surface influence on T-cell phenotype and function.

Shaikh Shimaz Hashimdeen1, Andy Römhild2, Michael Schmueck3

  • 1Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany.

Clinical Hemorheology and Microcirculation
|October 9, 2013
PubMed
Summary
This summary is machine-generated.

Culture chamber material and geometry significantly impact T lymphocyte proliferation and CD4/CD8 proportions. Polystyrene, polycarbonate, PSAN, and PEI supported T-cell growth, while PEU inhibited it, highlighting material selection importance for T-cell expansion systems.

Keywords:
T-cell culturegeometryimmunotherapymaterial testing

More Related Videos

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

15.5K
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

8.3K

Related Experiment Videos

Last Updated: May 7, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

52.8K
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

15.5K
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

8.3K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Limited information exists on T-cell interactions with culture systems.
  • Optimizing T-cell culture is crucial for immunotherapeutic applications.

Purpose of the Study:

  • To investigate the influence of culture chamber material and geometry on T-lymphocyte proliferation.
  • To assess the impact on CD4 and CD8 T-cell proportions.

Main Methods:

  • Tested five polymeric materials (PS, PC, PEU, PSAN, PEI) using injection molding.
  • Evaluated commercially available tissue culture plastic (TCP) well plates (24, 12, 6-well).
  • Stimulated T-lymphocytes using EBV peptide pools or MACS iBead particles in RPMI medium with supplements.

Main Results:

  • Four materials (PS, PC, PSAN, PEI) showed similar T-cell expansion with minimal impact on CD4/CD8 ratios.
  • Polyetherurethane (PEU) negatively affected T-cell growth and CD4/CD8 proportions.
  • Well plate geometry did not affect T-cell growth or maturation; smaller geometries favored initial proliferation.

Conclusions:

  • Culture system parameters, including material and geometry, significantly influence T-cell expansion.
  • Material selection is critical for maintaining T-cell function in immunotherapeutic applications.
  • Geometry influences T-cell proliferation, with smaller sizes being preferable initially.