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Updated: May 7, 2026

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
Published on: May 30, 2021
A conformation-switching fluorescent protein probe for detection of alpha synuclein oligomers
Michael Hernandez1, Yang Hu, Jin Ryoun Kim
1Department of Chemical and Biomolecular Engineering, Polytechnic Institute of NYU, 6 MetroTech Center, Brooklyn, NY 11201, USA. jkim@poly.edu.
Abstract:
We engineered the structural flexibility of an α synuclein (αS) variant, PG65, together with conformation-sensitive fluorescence to create a molecular probe for rapid, specific and quantitative detection of αS oligomers, which are the major toxic aggregate forms in Parkinson's disease.
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