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A cyanobenzo[a]phenoxazine-based near infrared lysosome-tracker for in cellulo imaging
1College of Chemistry, Chemical Engineering and Material Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, 199 Ren'Ai Road, Suzhou 215123, China. lujm@suda.edu.cn ge_jianfeng@hotmail.com.
Abstract:
A cyanobenzo[a]phenoxazine-based pH probe with pKa = 5.0 exhibits OFF-ON emission at 625-850 nm upon excitation at 600 nm in aqueous buffers. The in cellulo imaging experiments with HeLa cells indicate that the probe can serve as a lysosome-specific probe under red light excitation (633 nm) with near infrared emission (650-790 nm).

