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Published on: July 29, 2012
[Tryptase of mast cells in immune organs of developing chicken embryos]
Denghui Gao1, Junfeng Guo, Deyuan Ou
1College of Animal Sciences, Guizhou University, Guiyang 550025, China.
Objective:
To observe the form, distribution and emerging time of the mast cells (MCs) and tryptase positive cells (TPCs) in the immune organs of different days old chicken embryos.
Methods:
The thymus, bursa of Fabricius and spleen of chicken embryos at different development time were taken and fixed in Carnoy's solution. The alcian blue/safranine O (AB/SO) and streptavidin-biotin complex (SABC) immunohistochemistry were used to observe the MCs and TPCs.
Results:
MCs were first present in the thymus, bursa and spleen of day 13-14 embryos. The number of MCs increased along with the development days. On day 16, TPCs were first found in the immune organs. The form and distribution of TPCs were similar to MCs, but the emerging time of TPCs was later than that of MCs.
Conclusion:
Tryptase appears in the MCs in the immune organs of day 16 or later chicken embryos, and it is mainly located in the MCs of the connective tissues.
