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Updated: May 7, 2026
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Published on: June 6, 2016
[MicroRNA-10a accelerates endodermal lineage differentiation of mesenchymal stem cells from human placenta]
Dongmei Chen1, Haibin Ma, Shudan Liu
1Institute of Human Stem Cell, General Hospital, Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.
Objective:
To improve the potential of endodermal differentiation of human placenta-derived mesenchymal stem cells (PMSCs) by microRNA-10a (miR-10a)-mediated post-transcriptional regulation of its mRNA targets.
Methods:
Lentiviral vectors were used to stably and specifically over-express miR-10a and inhibited the miR-10a function by its antagomir. In addition, the relationship between miR-10a and Hoxa1 expression was analyzed. Real-time quantitative PCR (qRT-PCR) and immunofluorescent cytochemical staining were utilized to test the mRNA and protein expression variation and assess the ability of PMSCs to differentiate into endodermal cells. Results Over-expression of miR-10a led to the suppression of endogenous Hoxa1 expression, and inhibition of miR-10a relieved the repression of Hoxa1. Over-expression of miR-10a in PMSCs resulted in the up-regulation of endoderm-specific genes (FoxA2, Sox-17, Pdx-1 and Cdx2) and the increased proportions of FOXA2, SOX-17 and PDX-1 positive events as compared with the control treated cells.
Conclusion:
miR-10a was up-regulated during endodermal differentiation of PMSCs and involved in its differentiation partially via the suppression of the Hoxa1 gene. Furthermore, the miR-10a accelerates endodermal differentiation, likely mediated by the up-reguation of endoderm-specific down-stream genes.
