Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.4K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-throughput two-photon volumetric brain imaging in freely moving mice.

Nature communications·2025
Same author

Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

High-speed laser-scanning biological microscopy using FACED.

Nature protocols·2021
Same author

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

Nature methods·2020
Same author

Parallelized volumetric fluorescence microscopy with a reconfigurable coded incoherent light-sheet array.

Light, science & applications·2020
Same author

Flexible pulse-stretching for a swept source at 2.0  μm using free-space angular-chirp-enhanced delay.

Optics letters·2018

Related Experiment Video

Updated: May 7, 2026

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

17.8K

A fast fluorescence imaging flow cytometer for phytoplankton analysis.

Jianglai Wu, Robert K Y Chan

    Optics Express
    |October 10, 2013
    PubMed
    Summary

    A novel fluorescence imaging flow cytometer enables rapid phytoplankton analysis up to 1ml/min. This advanced instrument provides clear imaging for monitoring phytoplankton abundance and species composition in aquatic environments.

    Area of Science:

    • Oceanography
    • Biotechnology
    • Environmental Science

    Background:

    • Phytoplankton monitoring is crucial for aquatic ecosystem health.
    • Existing methods for phytoplankton analysis can be time-consuming and lack detailed imaging.
    • Rapid and accurate quantification of phytoplankton is needed for effective environmental management.

    Purpose of the Study:

    • To develop a fast fluorescence imaging flow cytometer for phytoplankton analysis.
    • To achieve high-resolution imaging with immunity to motion blur.
    • To assess the instrument's potential for field-based monitoring of phytoplankton.

    Main Methods:

    • Utilized thin light sheet illumination and image deconvolution to suppress out-of-focus light.
    • Implemented a unique flow configuration to prevent motion blur during image capture.

    More Related Videos

    Characterization of Aquatic Biofilms with Flow Cytometry
    08:30

    Characterization of Aquatic Biofilms with Flow Cytometry

    Published on: June 6, 2018

    8.8K
    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
    07:19

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

    Published on: June 28, 2017

    9.4K

    Related Experiment Videos

    Last Updated: May 7, 2026

    Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
    11:11

    Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

    Published on: August 15, 2013

    17.8K
    Characterization of Aquatic Biofilms with Flow Cytometry
    08:30

    Characterization of Aquatic Biofilms with Flow Cytometry

    Published on: June 6, 2018

    8.8K
    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
    07:19

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

    Published on: June 28, 2017

    9.4K
  • Achieved a lateral resolution of 0.75 ± 0.06 μm for particles ranging from 1 μm to 200 μm.
  • Operated at a volume flow rate of up to 1 ml/min.

    • Main Results:

    • The developed flow cytometer demonstrated high immunity to motion blur.
    • Successfully imaged phytoplankton across a wide size range (~1 μm to ~200 μm).
    • Preliminary analysis of coastal water samples showed promising results.

    Conclusions:

    • The fast fluorescence imaging flow cytometer is a promising tool for phytoplankton analysis.
    • The instrument has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.
    • This technology can significantly advance aquatic ecosystem monitoring capabilities.