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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Related Experiment Video

Updated: May 7, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective.

Hui Wang, Xiaoquan Yang, Yanyan Liu

    Optics Express
    |October 10, 2013
    PubMed
    Summary
    This summary is machine-generated.

    We created a new reflection-mode optical-resolution photoacoustic microscopy (OR-PAM) system for high-resolution imaging. This advanced OR-PAM offers a 1.2 μm lateral resolution and 0.9 mm penetration depth for studying microcirculation.

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    Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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    Area of Science:

    • Biomedical Optics
    • Photoacoustic Imaging
    • Microscopy

    Background:

    • Photoacoustic microscopy (PAM) is a hybrid imaging modality combining optical absorption contrast with ultrasound detection.
    • Reflection-mode PAM (R-PAM) offers advantages for deeper imaging but can suffer from signal degradation due to coupled optical and acoustic paths.
    • Developing advanced R-PAM systems is crucial for in vivo microcirculation studies.

    Purpose of the Study:

    • To develop and characterize a novel reflection-mode optical-resolution photoacoustic microscopy (OR-PAM) system.
    • To improve imaging resolution and working distance in R-PAM.
    • To demonstrate the system's capability for in vivo and in vitro microcirculation imaging.

    Main Methods:

    • A new R-PAM system was designed using a reflective objective for near diffraction-limited optical focusing.
    • An ultrasonic transducer was integrated into the central cone of the reflective objective for direct ultrasound detection.
    • The system's performance was evaluated for lateral resolution, penetration depth, and working distance.

    Main Results:

    • The developed OR-PAM system achieved a lateral resolution of 1.2 μm at 580 nm.
    • A penetration depth of 0.9 mm in biological tissues was demonstrated.
    • A working distance of 6.0 mm was achieved, and in vivo imaging of mouse ear microvasculature and in vitro imaging of red blood cells were successfully performed.

    Conclusions:

    • The novel R-PAM design effectively decouples optical focusing and ultrasound detection, enhancing imaging performance.
    • The system provides high resolution and sufficient penetration depth for microcirculation studies.
    • This advanced OR-PAM technology holds significant potential for preclinical research and biomedical applications.