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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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High sensitivity UV fluorescence spectroscopy based on an optofluidic jet waveguide.

Gianluca Persichetti, Genni Testa, Genni Gesta

    Optics Express
    |October 10, 2013
    PubMed
    Summary

    A new optofluidic liquid jet waveguide sensor detects water pollutants. This innovative spectroscopic sensor achieves very low detection limits for aromatic hydrocarbons and bacteria using autofluorescence.

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    Area of Science:

    • Spectroscopy
    • Optofluidics
    • Environmental Science

    Background:

    • Traditional water quality monitoring methods can be complex and time-consuming.
    • There is a need for sensitive and rapid detection techniques for water pollutants.

    Purpose of the Study:

    • To present a novel spectroscopic sensor utilizing an optofluidic liquid jet waveguide.
    • To demonstrate the sensor's capability in detecting common water pollutants.

    Main Methods:

    • A liquid jet waveguide was generated from the sample solution.
    • Total internal reflection was exploited to confine autofluorescence.
    • A self-aligned configuration coupled the liquid jet to a multimode optical fiber for detection.
    • UV excitation was used for fluorescence induction.

    Main Results:

    • The optofluidic liquid jet waveguide successfully confined autofluorescence.
    • Experimental measurements were performed on water solutions containing aromatic hydrocarbons and bacteria.
    • Very low limits of detection were achieved for the tested water pollutants.

    Conclusions:

    • The developed optofluidic liquid jet waveguide sensor is a promising tool for sensitive water quality monitoring.
    • The sensor offers a novel approach for the rapid detection of chemical and biological contaminants in water.