Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Competence-specific autolysis in Streptococcus sanguis.

D Horne, A Tomasz

    Journal of General Microbiology
    |March 1, 1985
    PubMed
    Summary

    Competent Streptococcus sanguis undergoes lysis due to a novel murein hydrolase, releasing intracellular components without degrading cell walls. This process is linked to genetic transformation competence.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Measurement of Reactor Antineutrino Oscillation at SNO.

    Physical review letters·2025
    Same author

    Transport and potential attenuation of nitrogen in shallow groundwaters in the lower Rangitikei catchment, New Zealand.

    Journal of contaminant hydrology·2017
    Same author

    Temporal and spatial water use on irrigated and nonirrigated pasture-based dairy farms.

    Journal of dairy science·2017
    Same author

    Water use on nonirrigated pasture-based dairy farms: Combining detailed monitoring and modeling to set benchmarks.

    Journal of dairy science·2017
    Same author

    Patterns of Pediatric Venous Insufficiency.

    Journal of vascular surgery. Venous and lymphatic disorders·2016
    Same author

    Hepatitis E in a Canadian traveller.

    The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Physiology

    Background:

    • Streptococcus sanguis, when competent for genetic transformation, exhibits a rapid decrease in optical density.
    • This phenomenon occurs upon transfer to an alkaline buffer with reducing agents, resembling autolysis.

    Purpose of the Study:

    • To investigate the mechanism behind the autolysis-like process in competent Streptococcus sanguis.
    • To identify the factors and cellular components involved in this rapid optical density decrease.

    Main Methods:

    • Studying the effect of preincubating the competence-inducing factor with an inactivating protein.
    • Analyzing the release of intracellular components (protein, RNA, lipid) during the process.
    • Assessing cell wall polymer integrity and detecting peptidoglycan hydrolase activity.

    Main Results:

    • The autolysis-like process was specific, as preventing competence induction also prevented lysis.
    • Significant release of intracellular protein, RNA, and lipid was observed.
    • No hydrolysis of phospholipids or degradation of cell wall polymers, including peptidoglycan, was detected.
    • No detectable peptidoglycan hydrolase activity against radiolabeled cell walls was found in unfractionated extracts.

    Conclusions:

    • Autolysis in competent Streptococcus sanguis is associated with the release of intracellular contents.
    • The process appears to involve a novel murein hydrolase with limited peptidoglycan bond-breaking activity.
    • This autolysis is specifically linked to the state of competence for genetic transformation.

    Related Experiment Videos