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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Identification of stage-specific breast markers using quantitative proteomics.

Sadr-ul Shaheed1, Nitin Rustogi, Andrew Scally

  • 1Institute of Cancer Therapeutics, University of Bradford , Tumbling Hill Street, Bradford BD7 1DP, United Kingdom.

Journal of Proteome Research
|October 11, 2013
PubMed
Summary

Quantitative proteomics identified key protein changes during breast cancer progression. Cofilin-1 and p23 show promise as potential biomarkers for invasive breast cancer, correlating with tumor grade and ER status, respectively.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Oncology

Background:

  • Breast cancer is a heterogeneous disease with varying stages of progression.
  • Understanding molecular alterations is crucial for identifying diagnostic and prognostic markers.

Purpose of the Study:

  • To identify protein expression changes associated with breast cancer progression using quantitative proteomics.
  • To validate potential protein biomarkers for breast cancer.

Main Methods:

  • Quantitative proteomics analysis of matched normal and tumor tissues (fibroadenoma, DCIS, invasive ductal carcinoma).
  • iTRAQ labeling and mass spectrometry for protein identification and quantification.
  • Bioinformatics analysis for pathway enrichment and protein network identification.
  • Western blotting, multiple reaction monitoring mass spectrometry, and tissue microarray analysis for validation.

Main Results:

  • 59 proteins were significantly altered in tumor tissues compared to normal tissues, with most changes in invasive carcinomas.
  • Bioinformatics analysis revealed altered proteins involved in redox regulation, lipid transport, protein folding, and proteasomal degradation, with Myc oncogene activation implicated.
  • Cofilin-1 and p23 were increased, while membrane copper amine oxidase 3 was decreased in invasive carcinoma.
  • Cofilin-1 and p23 showed positive correlations with tumor grade and ER-positive status, respectively, in a large patient cohort.

Conclusions:

  • Proteomic profiling reveals significant protein alterations during breast cancer progression.
  • Cofilin-1 and p23 are potential biomarkers for breast cancer, warranting further investigation for clinical utility.