Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

35.4K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
35.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using LLM-generated tools to extract information about reporting statistical software in biomedical and health science research articles.

BMC research notes·2026
Same author

Multiple Confabulations Found in Bioinformatics Tasks Carried Out by Several Free Large Language Models.

Current genomics·2026
Same author

Automated analyses of risk of bias and critical appraisal of systematic reviews (ROBIS and AMSTAR 2): a comparison of the performance of 4 large language models.

Journal of the American Medical Informatics Association : JAMIA·2025
Same author

Transferability of European-derived Alzheimer's disease polygenic risk scores across multiancestry populations.

Nature genetics·2025
Same author

Large Language Models and the Analyses of Adherence to Reporting Guidelines in Systematic Reviews and Overviews of Reviews (PRISMA 2020 and PRIOR).

Journal of medical systems·2025
Same author

DNA methylation in peripheral blood leukocytes in late onset Alzheimer's disease.

Journal of Alzheimer's disease reports·2025

Related Experiment Video

Updated: May 7, 2026

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

21.7K

Optimizing methodologies for PCR-based DNA methylation analysis.

Hernán G Hernández1, M Yat Tse, Stephen C Pang

  • 1Laboratory of NeuroPsychiatric Genetics, Biomedical Sciences Research Group, School of Medicine, Universidad Antonio Nariño, Bogotá, Colombia; Neurosciences Research Group, School of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia; Biomedical Sciences Doctoral Program, School of Medicine, Universidad Nacional de Colombia, Bogotá, Colombia.

Biotechniques
|October 11, 2013
PubMed
Summary
This summary is machine-generated.

Understanding DNA methylation is key to human disease research. This review guides researchers through common PCR-based methods like bisulfite sequencing PCR (BSP) and methylation-specific PCR (MSP), aiding in technique selection.

More Related Videos

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

108.9K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.3K

Related Experiment Videos

Last Updated: May 7, 2026

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

21.7K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

108.9K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • DNA methylation analysis is crucial for understanding human diseases.
  • Numerous PCR-based DNA methylation studies are published annually.
  • Researchers new to methylation analysis face challenges in method selection and implementation.

Purpose of the Study:

  • To review common PCR-based DNA methylation analysis techniques.
  • To provide a critical analysis of the strengths and weaknesses of each method.
  • To offer guidelines for selecting and implementing appropriate methylation analysis methods.

Main Methods:

  • Bisulfite sequencing PCR (BSP)
  • Methylation-specific PCR (MSP)
  • MethyLight
  • Methylation-sensitive high-resolution melting (MS-HRM)

Main Results:

  • Each method (BSP, MSP, MethyLight, MS-HRM) has distinct advantages and limitations.
  • Guidelines are provided to aid researchers in choosing the most suitable technique.
  • The review facilitates informed decision-making for DNA methylation studies.

Conclusions:

  • Selecting the correct PCR-based DNA methylation analysis technique is vital for research success.
  • This review serves as a practical resource for molecular genetics researchers.
  • Effective implementation of these methods enhances the understanding of disease mechanisms.