Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

46.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swine reporter model for preclinical evaluation and characterization of gene delivery vectors.

Molecular therapy. Advances·2026
Same author

Midgestational injection of highly expanded human CD34+ cells increases lineages of human immune cells and supports thymic development in <i>RAG2-/-IL2RG-/Y</i> SCID pigs.

Frontiers in immunology·2026
Same author

LC-MS measurement of triglyceride hydrolysis: Application in studies of Patatin-like phospholipase domain-containing protein 3 activity.

Analytical biochemistry·2026
Same author

Light stress regulates pigment concentrations, growth, and gene expression in hatchery-cultivated Kappaphycus alvarezii (Solieriaceae, Rhodophyta).

Journal of phycology·2026
Same author

Development of a large porcine model of osteogenesis imperfecta type I.

Bone reports·2026
Same author

WRN structural flexibility showcased through fragment-based lead discovery of inhibitors.

Nature communications·2026

Related Experiment Video

Updated: May 7, 2026

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
08:48

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

5.3K

Live pigs produced from genome edited zygotes.

Simon G Lillico1, Chris Proudfoot, Daniel F Carlson

  • 1The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.

Scientific Reports
|October 11, 2013
PubMed
Summary

Genome editing technologies like Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) can now create live, genome-edited pigs from zygotes. This breakthrough enables gene knockout in livestock, expanding genome editing applications.

More Related Videos

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.1K
Author Spotlight: Surgical Methods and Outcomes in Oviductal Cloned Pig Embryo Transfers
05:41

Author Spotlight: Surgical Methods and Outcomes in Oviductal Cloned Pig Embryo Transfers

Published on: October 18, 2024

1.6K

Related Experiment Videos

Last Updated: May 7, 2026

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
08:48

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Published on: June 24, 2022

5.3K
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.1K
Author Spotlight: Surgical Methods and Outcomes in Oviductal Cloned Pig Embryo Transfers
05:41

Author Spotlight: Surgical Methods and Outcomes in Oviductal Cloned Pig Embryo Transfers

Published on: October 18, 2024

1.6K

Area of Science:

  • Agricultural Science
  • Genetics and Genomics
  • Biotechnology

Background:

  • Site-directed genome engineering is crucial for livestock improvement.
  • Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) are established genome editing tools.

Purpose of the Study:

  • To demonstrate the direct application of TALEN and ZFN for genome editing in pig zygotes.
  • To establish a method for producing live, genome-edited pigs using these technologies.

Main Methods:

  • Direct injection of TALEN and ZFN into pig zygotes.
  • Analysis of resulting pig embryos and live-born pigs for genome editing events.

Main Results:

  • Successful production of live genome-edited pigs following TALEN and ZFN injection into zygotes.
  • Identification of monoallelic, heterozygous, and homozygous biallelic editing events.
  • Demonstration of the feasibility of gene knockout in pig zygotes from various mating types.

Conclusions:

  • TALEN and ZFN can be effectively applied directly to pig zygotes to generate live, genome-edited offspring.
  • This method significantly broadens the utility of genome editing technologies in livestock breeding.
  • The ability to achieve various biallelic events facilitates diverse genetic modifications in pigs.