YAP and TAZ regulate skin wound healing.
Min-Jung Lee1, Mi Ran Byun2, Makoto Furutani-Seiki3
1Division in Anatomy and Developmental Biology, Department of Oral Biology, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
The Journal of Investigative Dermatology
|October 11, 2013
Summary
Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are crucial for skin wound healing. Their nuclear localization is essential for timely wound closure and regulating key signaling pathways.
Area of Science:
- Cell Biology
- Dermatology
- Regenerative Medicine
Background:
- The Hippo signaling pathway is vital for organ size and tissue regeneration.
- Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are key downstream effectors of this pathway.
- The specific roles of YAP and TAZ in skin wound healing are not well understood.
Purpose of the Study:
- To investigate the necessity of YAP and TAZ in cutaneous wound healing.
- To determine the impact of YAP and TAZ on the skin wound healing process.
Main Methods:
- Small interfering RNA (siRNA)-mediated knockdown of YAP and TAZ in full-thickness skin wounds.
- Analysis of YAP and TAZ expression patterns in wounded skin.
- Assessment of wound closure rates and expression of related signaling molecules.
Main Results:
- YAP and TAZ show dynamic nuclear localization in wounded skin.
- Knockdown of YAP and TAZ significantly delayed wound closure.
- YAP/TAZ inhibition reduced transforming growth factor-β1 (TGF-β1) expression and modulated TGF-β1 pathway components (Smad-2, p21, Smad-7).
Conclusions:
- Nuclear localization of YAP and TAZ is essential for effective skin wound healing.
- YAP and TAZ play a critical role in regulating TGF-β1 signaling during wound repair.
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