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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
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Molecular diagnosis of Actinomadura madurae infection by 16S rRNA deep sequencing
Stephen J Salipante1, Dhruba J Sengupta, Daniel R Hoogestraat
1Departments of Laboratory Medicine.
Journal of Clinical Microbiology
|October 11, 2013
Abstract:
Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.
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