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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
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Steady-state fluorescence polarization/anisotropy for the study of protein interactions
Nicholas G James1, David M Jameson
1Department Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2013
Abstract:
Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein-ligand complex.

