Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Importance of Riboswitch-regulated Genes for Pseudomonas aeruginosa.

Journal of molecular biology·2026
Same author

Molecular Insights Into the Binding Dynamics of Transcription Factor TBR1 to T-box DNA Sequences.

Journal of molecular biology·2025
Same author

The Zuo1 C-terminal domain stabilizes DNA guanosine quadruplex (G4) structures located on Chromosome IX in Saccharomyces cerevisiae.

Nucleic acids research·2025
Same author

Inherited chromosomally integrated human herpesvirus 6: regional variation in prevalence, association with angina, and identification of ancestral viral lineages in two large UK studies.

Journal of virology·2025
Same author

The Thiamin Pyrophosphate Riboswitch is Affected by Lysine-derived Metabolic Conversion Products in Escherichia coli.

Journal of molecular biology·2025
Same author

Insights into the cotranscriptional and translational control mechanisms of the Escherichia coli tbpA thiamin pyrophosphate riboswitch.

Communications biology·2024

Related Experiment Video

Updated: May 7, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

12.7K

Single-molecule fluorescence of nucleic acids.

Kaley McCluskey1, Euan Shaw, Daniel A Lafontaine

  • 1SUPA School of Physics and Astronomy, University of St. Andrews, St. Andrews, Scotland, UK.

Methods in Molecular Biology (Clifton, N.J.)
|October 11, 2013
PubMed
Summary

Single-molecule fluorescence techniques, particularly single-molecule Förster Resonance Energy Transfer (smFRET), offer unparalleled insights into nucleic acid dynamics and function. These methods reveal complex cellular mechanisms by analyzing individual DNA and RNA molecules without synchronization.

More Related Videos

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

1.9K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

1.7K

Related Experiment Videos

Last Updated: May 7, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

12.7K
Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

1.9K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

1.7K

Area of Science:

  • Biochemistry and Molecular Biology
  • Biophysics
  • Genetics

Background:

  • Single-molecule fluorescence studies are transforming the understanding of nucleic acid processing.
  • Techniques provide detailed molecular insights into DNA repair, replication, transcription, and RNA folding.
  • Unmatched ability to resolve heterogeneous populations and transient states drives technique growth.

Purpose of the Study:

  • To introduce the practical aspects of applying single-molecule techniques to nucleic acid research.
  • To highlight the advantages of single-molecule fluorescence for studying structure and dynamics.
  • To enable broader implementation of these advanced methods.

Main Methods:

  • Single-molecule fluorescence, with a focus on single-molecule Förster Resonance Energy Transfer (smFRET).
  • Leveraging advances in nucleic acid synthesis, labeling, and immobilization.
  • Utilizing improved dye photophysics and standardized analysis methods.

Main Results:

  • Demonstration of resolving heterogeneous static and dynamic populations.
  • Identification of transient and low-populated states in nucleic acid processes.
  • Enabling single-molecule techniques beyond specialized research settings.

Conclusions:

  • Single-molecule fluorescence is revolutionizing nucleic acid research.
  • Practical implementation is becoming more accessible due to technological advancements.
  • These techniques are crucial for understanding fundamental cellular mechanisms.