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Fluorescent protein capped mesoporous nanoparticles for intracellular drug delivery and imaging
Xinjian Yang1, Zhenhua Li, Meng Li
1State Key Laboratory of Rare Earth Resource Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 (P.R. China), Fax: (+86) 0431-85262625; Graduate School of the Chinese Academy of Sciences, Beijing, 100039 (P.R. China).
Abstract:
A multifunctional system for intracellular drug delivery and simultaneous fluorescent imaging was constructed by using histidine-tagged, cyan fluorescent protein (CFP)-capped magnetic mesoporous silica nanoparticles (MMSNs). This protein-capped multifunctional nanostructure is highly biocompatible and does not affect cell viability or proliferation. The CFP acts not only as a capping agent, but also as a fluorescent imaging agent. The nanoassembly was activated by histidine-based replacement, leading to release of drug molecules encapsulated in the nanopores into the bulk solution. The fluorescent imaging functionality would allow noninvasive tracking of the nanoparticles in the body. By combining the drug delivery with cell-imaging capability, these nanoparticles may provide valuable multifunctional nanoplatforms for biomedical applications.
