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Updated: May 7, 2026

15:57
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Published on: October 9, 2009
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Metallic tip enhanced fluorescence for DNA replication monitoring
Abstract:
We have successfully performed localized loop-mediated isothermal reactions of hepatitis B virus (HBV) and hepatitis C virus (HCV) on the apex (50~100 nm) of metallic tips coated with Bst polymerases. The SYBR green molecules binding to the new formed HBV DNA inside the optical near fields were excited by two-photon fluorescence microscopy, and directly imaged in far field. Another reporter primer is used for HCV replication detection. Preliminary results are presented in this manuscript.

