Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support

Area of Science:

• Oncology
• Biotechnology
• Medical Diagnostics

Background:

• Frozen section analysis is crucial for tumor assessment but can be subjective.
• A quantitative method for intraoperative tumor grading is needed to complement histopathology.
• Previous work introduced intraoperative flow cytometry (iFC) to aid frozen section examination.

Purpose of the Study:

• To develop and evaluate an advanced automated system for rapid DNA ploidy analysis.
• To assess the system's utility in intraoperative glioma diagnosis and grading.
• To provide a quantitative measure of tumor malignancy during surgery.

Main Methods:

• Development of a "Fully Automatic Rapid DNA Ploidy Analyzer" with tissue pretreatment and freeze-dried reagents.
• Analysis of glioma patient biopsy samples during open surgery.
• Calculation of Malignancy Index (MI) and correlation with histological grade (WHO).

Main Results:

• Significant difference in Malignancy Index (MI) between neoplastic and perilesional brain tissue (26.0% vs 4.1%, P<0.001).
• Optimal cut-off MI of 6.8% achieved 86% sensitivity and 81% specificity for tumor identification.
• Good correlation observed between MI and WHO histological grade.
• Complete analysis from sample preparation to result in under ten minutes.

Conclusions:

• The automated DNA ploidy analyzer is a feasible tool for rapid glioma detection in surgical biopsies.
• The system provides quantitative data (MI) that correlates with tumor malignancy and histological grade.
• This technology enhances intraoperative decision-making by offering rapid, objective tumor assessment.