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Updated: May 7, 2026

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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Electroporation and electrofusion in field-tailored microstructures.

Masao Washizu

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 11, 2013
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    Summary

    Electrical pulses create temporary cell membrane pores for introducing substances or fusing cells. Micro-fabricated devices enable precise control for efficient, low-invasive cell poration and fusion, including gene transfection and cytoplasmic transplants.

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    Area of Science:

    • Biotechnology
    • Cell Biology
    • Bioengineering

    Background:

    • Introducing foreign substances into cells is fundamental for cell engineering.
    • Electrical pulses can reversibly permeabilize cell membranes, creating transient pores.
    • These pores allow molecule diffusion into cells or cell fusion.

    Purpose of the Study:

    • To review research on utilizing electrical pulses and micro-fabricated structures for cell engineering.
    • To demonstrate precise control over single-cell manipulation, membrane voltage, and pore formation.
    • To highlight applications in gene transfection, electrofusion, and cytoplasmic transplantation.

    Main Methods:

    • Employing micro-fabricated structures with dimensions smaller than cells.
    • Designing and tailoring electrical field patterns for controlled cell manipulation.
    • Applying electrical pulses to induce reversible membrane poration and cell fusion.

    Main Results:

    • Achieved low-invasive, high-yield poration and fusion of single cells.
    • Demonstrated precise control over the location and magnitude of membrane voltage.
    • Successfully applied the technique for gene transfection, electrofusion, and cytoplasmic transplant.

    Conclusions:

    • Micro-fabricated devices offer precise control for advanced cell engineering techniques.
    • Electrical poration and fusion are effective methods for genetic material and cytoplasm transfer.
    • This approach enables sophisticated manipulation of cellular processes with high efficiency.