Urine glucose analysis with functionalized graphene oxide as a material for amperometric sensor

Area of Science:

• Electrochemistry
• Materials Science
• Biomedical Engineering

Background:

• Urine glucose monitoring is crucial for managing diabetes and other health conditions.
• Existing methods for urine glucose detection can be complex or lack sensitivity.
• Graphene oxide (GO) offers unique electrochemical properties for biosensing applications.

Purpose of the Study:

• To develop and evaluate a novel functionalized graphene oxide (FGO) sensor for accurate glucose detection in urine.
• To assess the sensor's performance characteristics, including linearity, sensitivity, and stability.
• To demonstrate the practical applicability of the FGO sensor for real-world urine glucose analysis.

Main Methods:

• Fabrication of a gold-printed circuit board (Au-PCB) substrate.
• Systematic coating of functionalized graphene oxide (FGO) onto the Au-PCB.
• Electrochemical measurements to determine the sensor's response to varying glucose concentrations.
• Validation of the sensor's performance using human urine samples.

Main Results:

• The FGO-Au-PCB sensor exhibited a wide linearity range for glucose detection from 1.7 to 44.4 mM.
• Sensor measurements showed high stability, with constant variables less than 3% across multiple trials.
• The sensor successfully detected glucose in human urine samples, demonstrating practical utility.
• Urine glucose levels measured by the sensor showed no significant difference compared to blood glucose levels from the same individuals.

Conclusions:

• The developed FGO-Au-PCB sensor provides a reliable and sensitive method for urine glucose detection.
• The sensor's performance in urine samples suggests its potential as a non-invasive diagnostic tool.
• This technology offers a promising advancement for convenient and accurate glucose monitoring in clinical and personal health management.