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Evolved Quorum sensing regulator, LsrR, for altered switching functions.

Bryn L Adams1, Karen K Carter, Min Guo

  • 1Fischell Department of Bioengineering, ‡Institute for Bioscience and Biotechnology Research, §Department of Chemical and Biomolecular Engineering, and ∥Department of Chemistry and Biochemistry, University of Maryland , College Park, Maryland 20742 United States .

ACS Synthetic Biology
|October 12, 2013
PubMed
Summary
This summary is machine-generated.

Researchers engineered novel synthetic biology tools, the eLsrR and aLsrR switches, to control gene expression in bacteria using quorum sensing (QS) signals for advanced microbial applications.

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Area of Science:

  • Synthetic Biology
  • Microbial Communication
  • Biotechnology

Background:

  • Cell-to-cell communication is crucial for coordinating functions in microbial populations.
  • Quorum sensing (QS) is a conserved bacterial communication mechanism.
  • Engineered QS systems offer powerful tools for synthetic biology.

Purpose of the Study:

  • To expand the utility of the E. coli LsrR regulator in synthetic biology.
  • To develop novel LsrR-based synthetic switches with enhanced or reversed functions.
  • To enable precise control of gene expression in engineered microbial consortia.

Main Methods:

  • Directed evolution was used to generate two novel LsrR variants: eLsrR and aLsrR.
  • Protein modeling and docking studies were performed to understand signal binding.
  • A coculture system demonstrated the function of the aLsrR switch for inter-strain communication.

Main Results:

  • Two novel LsrR switches, eLsrR (enhanced repression/derepression) and aLsrR (activator), were successfully created.
  • Protein modeling provided insights into the mechanism of QS signal interaction with the evolved proteins.
  • The aLsrR switch enabled AI-2 controlled, strain-specific gene expression inhibition in a coculture system.

Conclusions:

  • Novel synthetic biology tools (eLsrR and aLsrR) were developed based on the E. coli LsrR regulator.
  • These tools allow for tunable, simultaneous control of gene expression in distinct microbial populations via QS.
  • This work advances the development of engineered microbial consortia for complex synthetic functions and products.