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Updated: May 7, 2026

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Toward single-molecule microscopy on a smart phone.

Saumyakanti Khatua1, Michel Orrit

  • 1Leiden Institute of Physics, Universiteit Leiden , 2300 RA Leiden, The Netherlands.

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|October 12, 2013
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Summary

Researchers developed a portable, cost-effective fluorescence imaging device capable of visualizing single nano-objects, including large viruses. This breakthrough enables single-molecule microscopy and spectroscopy applications on smartphones.

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Area of Science:

  • Nanotechnology
  • Optical Microscopy
  • Spectroscopy

Background:

  • Fluorescence imaging allows visualization of single nano-objects and fluorophores.
  • Current fluorescence microscopy is limited to expensive, lab-based instrumentation.

Purpose of the Study:

  • To develop a sensitive, cost-effective, and portable device for imaging individual nano-objects.
  • To explore the potential of smartphone-based single-molecule microscopy and spectroscopy.

Main Methods:

  • Development of a novel, portable fluorescence imaging device.
  • Demonstration of imaging capabilities for nano-objects as small as large viruses.

Main Results:

  • Successful imaging of individual nano-objects using the portable device.
  • Achieved sensitivity comparable to traditional fluorescence microscopy.

Conclusions:

  • The developed device democratizes advanced nanoscale imaging.
  • Opens new avenues for portable single-molecule analysis and applications in various scientific fields.