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Detecting Salmonella serovars in shell eggs by loop-mediated isothermal amplification.

Qianru Yang1, Siyi Chen, Beilei Ge

  • 1Department of Food Science, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA.

Journal of Food Protection
|October 12, 2013
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Summary

Two loop-mediated isothermal amplification (LAMP) assays offer rapid, accurate detection of Salmonella in shell eggs. These methods are significantly more sensitive than PCR, improving food safety and public health outcomes.

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Area of Science:

  • Food Science
  • Microbiology
  • Public Health

Background:

  • Shell eggs contaminated with Salmonella Enteritidis present significant food safety risks.
  • Rapid, accurate, and reliable detection methods for Salmonella are crucial for enhanced product testing.
  • Existing methods like PCR have limitations in speed and sensitivity for Salmonella detection in eggs.

Purpose of the Study:

  • To compare the performance of two loop-mediated isothermal amplification (LAMP) assays against PCR.
  • To evaluate the detection limits and sensitivity of LAMP assays for Salmonella Enteritidis and Salmonella Typhimurium in egg homogenates.
  • To assess the suitability of LAMP assays for routine Salmonella testing in shell eggs.

Main Methods:

  • Two loop-mediated isothermal amplification (LAMP) assays targeting the Salmonella invasion protein (invA) gene were utilized.
  • The performance of LAMP assays was compared with Polymerase Chain Reaction (PCR).
  • Experiments involved detecting Salmonella serovars in pure culture and experimentally contaminated egg homogenates, with and without enrichment.

Main Results:

  • Both LAMP assays demonstrated high specificity and were approximately 100-fold more sensitive than PCR in pure culture.
  • LAMP assays detected Salmonella down to 10(4) CFU/25 ml without enrichment and 10(0) CFU/25 ml with 8-h enrichment in egg homogenates.
  • PCR required significant enrichment (12-24 h) and was less sensitive, failing to detect Salmonella below 10(6) CFU/25 ml directly.

Conclusions:

  • The evaluated LAMP assays are rapid, accurate, and reliable for detecting Salmonella serovars in shell eggs.
  • LAMP assays offer superior sensitivity and reduced time compared to PCR for Salmonella detection in eggs.
  • These LAMP assays hold potential for adoption in routine egg testing to enhance food safety and protect public health.