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Updated: May 7, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
[Interest of the study of a not identified peak on a hemoglobin chromatogram: delta variant or inter-sample
Aurore Desmons1, Stéphane Jaisson, Philippe Gillery
1Laboratoire de biologie et de recherche pédiatriques, American Memorial Hospital, CHU de Reims, France.
Abstract:
D-10(®) (Bio-Rad) analyzer using cationic exchange high performance chromatography (HPLC) allows the detection of the main hemoglobin variants. This observation shows the presence of a peak on chromatogram with a low intensity and no quantifiable which can lead to different diagnosis. Inter-sample contaminations can be confused with the presence of an hemoglobin variant. This case highlights the importance of the knowledge of technicals limits for validation and clinical use of results.

