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Updated: May 7, 2026
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
Published on: November 14, 2018
Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of
Yanwei Jia1, Pui-In Mak, Conner Massey
1Department of Biology, Brandeis University, 415 South Street, Waltham, MA 02454, USA. wangh@brandeis.edu.
Abstract:
LATE-PCR is an advanced form of non-symmetric PCR that efficiently generates single-stranded DNA which can readily be characterized at the end of amplification by hybridization to low-temperature fluorescent probes. We demonstrate here for the first time that monoplex and duplex LATE-PCR amplification and probe target hybridization can be carried out in double layered PDMS microfluidics chips containing dried reagents. Addition of a set of reagents during dry down overcomes the common problem of single-stranded oligonucleotide binding to PDMS. These proof-of-principle results open the way to construction of inexpensive point-of-care devices that take full advantage of the analytical power of assays built using LATE-PCR and low-temperature probes.
