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Updated: May 7, 2026
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
Published on: March 6, 2018
High-efficiency localization of Na(+)-K(+) ATPases on the cytoplasmic side by direct stochastic optical
Jiazhen Wu1, Jing Gao, Miao Qi
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China. hdwang@ciac.ac.cn jiangjg@ciac.ac.cn.
Abstract:
We describe a concise and effective strategy towards precisely mapping Na(+)-K(+) ATPases on the cytoplasmic side of cell membranes by direct stochastic optical reconstruction microscopy (dSTORM). We found that most Na(+)-K(+) ATPases are localized in different sizes of clusters on human red blood cell (hRBC) membranes, revealed by Ripley's K-function analysis. Further evidence that cholesterol depletion causes the dispersion of Na(+)-K(+) ATPase clusters indicates that such clusters could be localized in cholesterol-enriched domains. Our results suggest that Na(+)-K(+) ATPases might aggregate within the lipid rafts to fulfill their functions.
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