Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypoxia01:23

Hypoxia

2.4K
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
2.4K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

911
Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
911

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Applications and prospects of artificial intelligence for decision-making in spinal surgery].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2026
Same author

[Inflammatory bowel disease increases the risk of hearing loss via autoimmunity: a Mendelian randomization study].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2026
Same author

Isomer Depletion of ^{93m}Mo Triggered by Inelastic Nuclear Scattering Rather than Nuclear Excitation by Electron Capture.

Physical review letters·2026
Same author

[Adenovirus-induced liver failure in adults following allogeneic hematopoietic stem cell transplantation: a report of two cases and literature review].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2026
Same author

[Analysis of herpes zoster vaccination intention and related factors among ≥50-year-old patients with multiple chronic diseases in Zhejiang Province].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

[Effects of ultrathin inguinal flap free transplantation in repairing hand and foot wounds].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2026

Related Experiment Video

Updated: May 7, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

7.6K

Expression profiling analysis of hypoxic pulmonary disease.

L Zhou1, L M Wang, H M Song

  • 1Department of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Genetics and Molecular Research : GMR
|October 12, 2013
PubMed
Summary

Low oxygen levels cause pulmonary hypertension. This study identified key genes and potential drugs like pindolol for treating hypoxia-related lung diseases.

More Related Videos

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

17.4K
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
10:15

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Published on: January 7, 2019

7.2K

Related Experiment Videos

Last Updated: May 7, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
09:45

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

7.6K
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

17.4K
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
10:15

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Published on: January 7, 2019

7.2K

Area of Science:

  • Pulmonary Medicine
  • Molecular Biology
  • Genomics

Background:

  • Environmental hypoxia can lead to chronic pulmonary hypertension and vascular changes in the lungs.
  • Understanding the molecular mechanisms of hypoxia in lung microvascular endothelial cells is crucial for developing effective treatments.

Purpose of the Study:

  • To identify differentially expressed genes in human lung microvascular endothelial cells under hypoxic conditions.
  • To explore the functional roles of these genes using gene ontology analysis.
  • To identify potential therapeutic small molecules by comparing gene expression data with the Connectivity Map.

Main Methods:

  • Utilized the microarray dataset GSE11341 to analyze gene expression changes.
  • Performed gene ontology term enrichment analysis to understand gene functions.
  • Integrated Connectivity Map analysis to identify potential drug candidates.

Main Results:

  • Identified 22, 21, and 29 differentially expressed genes at 3, 24, and 48 hours of hypoxia, respectively.
  • Six key genes (ADM, HMOX1, VEGFA, EGLN3, APOLD1, ANGPTL4) were significantly associated with the cellular response to hypoxia.
  • Identified three potential therapeutic agents: pindolol, sulfapyridine, and ciclopirox.

Conclusions:

  • The study identified critical genes involved in the response to pulmonary microvascular endothelial cell hypoxia.
  • Pindolol, sulfapyridine, and ciclopirox emerged as promising candidates for treating hypoxia-induced pulmonary diseases.
  • These findings offer potential drug targets for clinical intervention in patients with hypoxic pulmonary conditions.