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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

4.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.8K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Related Experiment Video

Updated: May 7, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
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Developments in coupled solid-phase extraction-capillary electrophoresis 2011-2013.

Rawi Ramautar1, Govert W Somsen, Gerhardus J de Jong

  • 1Leiden Academic Center for Drug Research, Leiden University, Leiden, the Netherlands.

Electrophoresis
|October 12, 2013
PubMed
Summary

This review details coupled Solid Phase Extraction-Capillary Electrophoresis (SPE-CE) systems from 2011-2013. It highlights advancements like frit-free and chip-based SPE for enhanced sample analysis.

Keywords:
Biomedical and pharmaceutical applicationsEnvironmental applicationsIn-line SPE-CEOn-chip SPEOn-line SPE-CE

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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Last Updated: May 7, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Solid Phase Extraction-Capillary Electrophoresis (SPE-CE) is a powerful hyphenated technique.
  • Previous reviews covered SPE-CE up to 2011.

Purpose of the Study:

  • To provide an updated overview of SPE-CE systems reported between January 2011 and June 2013.
  • To describe in-line and on-line SPE-CE approaches.
  • To outline emerging technological developments in SPE-CE.

Main Methods:

  • Literature review of SPE-CE systems.
  • Focus on in-line and on-line coupling strategies.
  • Inclusion of novel techniques like frit-free and chip-based SPE.

Main Results:

  • Comprehensive overview of SPE-CE applications in biomedical, pharmaceutical, and environmental analysis.
  • Detailed table of recent SPE-CE studies including sample type, sorbent, coupling, detection, and LOD.
  • Identification of emerging trends and technological advancements.

Conclusions:

  • SPE-CE continues to evolve with new technologies.
  • The technique demonstrates broad applicability across various analytical fields.
  • Future perspectives for SPE-CE development are discussed.