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Updated: May 7, 2026

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
A fluorogenic probe for γ-glutamyl cyclotransferase: application of an enzyme-triggered O-to-N acyl migration-type
Taku Yoshiya1, Shugo Tsuda, Masayoshi Mochizuki
1SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan). t.yoshiya@peptide.co.jp.
Abstract:
Light it up: human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA-4, which should contribute toward further understanding of GGCT.
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