Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
Bioreactor Controls-III01:22

Bioreactor Controls-III

71
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
71
Microbes in Food Production01:29

Microbes in Food Production

409
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
409
Microbial Fermentation01:23

Microbial Fermentation

1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Fates of Pyruvate01:20

Fates of Pyruvate

9.1K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acetate Shock Loads Enhance CO Uptake Rates of Anaerobic Microbiomes.

Microbial biotechnology·2024
Same author

Modulation of starch-based film properties for encapsulation of microbial inoculant.

International journal of biological macromolecules·2024
Same author

Carboxymethylcellulose production from sugarcane bagasse: A new approach in biorefinery concept.

International journal of biological macromolecules·2024
Same author

Two-stage conversion of syngas and pyrolysis aqueous condensate into L-malate.

Biotechnology for biofuels and bioproducts·2024
Same author

Mechanochemical recycling of cellulose multilayer carton packages to produce micro and nanocellulose from the perspective of techno-economic and environmental analysis.

Journal of environmental management·2024
Same author

Formate-induced CO tolerance and methanogenesis inhibition in fermentation of syngas and plant biomass for carboxylate production.

Biotechnology for biofuels and bioproducts·2023

Related Experiment Video

Updated: May 7, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

1.9K

Saccharification of biomass using whole solid-state fermentation medium to avoid additional separation steps.

Rosangela D P B Pirota1, Flávio C F Baleeiro, Cristiane S Farinas

  • 1Programa de Pós-graduação em Biotecnologia, Universidade Federal de São Carlos, 13565-905, São Carlos, SP, Brazil; Embrapa Instrumentação, Rua XV de Novembro 1452, 13560-970, São Carlos, SP, Brazil.

Biotechnology Progress
|October 12, 2013
PubMed
Summary
This summary is machine-generated.

Enzymatic hydrolysis of sugarcane bagasse using whole fermentation media from Aspergillus niger is more effective than Trichoderma reesei. Eliminating enzyme extraction steps reduces costs and waste, aligning with biorefinery principles.

Keywords:
Aspergillus nigerTrichoderma reeseibiorefinerycellulasesenzymatic hydrolysissaccharificationsolid-state fermentationsugarcane bagassewhole medium

More Related Videos

High-throughput Saccharification Assay for Lignocellulosic Materials
11:39

High-throughput Saccharification Assay for Lignocellulosic Materials

Published on: July 3, 2011

16.8K
Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
07:34

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production

Published on: June 15, 2014

27.0K

Related Experiment Videos

Last Updated: May 7, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

1.9K
High-throughput Saccharification Assay for Lignocellulosic Materials
11:39

High-throughput Saccharification Assay for Lignocellulosic Materials

Published on: July 3, 2011

16.8K
Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
07:34

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production

Published on: June 15, 2014

27.0K

Area of Science:

  • Biotechnology
  • Biomass Conversion
  • Enzymology

Background:

  • Sugarcane bagasse is a lignocellulosic biomass rich in cellulose.
  • Efficient enzymatic hydrolysis is crucial for converting biomass into valuable products.
  • Current methods often involve costly enzyme extraction and purification steps.

Purpose of the Study:

  • To compare the efficacy of enzymatic hydrolysis of steam-exploded sugarcane bagasse (SESB) using different enzyme preparations.
  • To evaluate the potential of using whole fermentation media (WM) to reduce process costs.
  • To assess the compatibility of the process with biorefinery concepts.

Main Methods:

  • Solid-state fermentation (SSF) of wheat bran using Aspergillus niger and Trichoderma reesei to produce enzyme preparations.
  • Enzymatic hydrolysis of SESB using in-house enzymatic extracts (EE) and WM.
  • Quantitative analysis of total reducing sugars (TRS) released over time using the Chrastil model.
  • Comparison of hydrolysis yields under various conditions, including supplementation with commercial enzymes.

Main Results:

  • Aspergillus niger enzymatic complex achieved up to 3.2-fold higher SESB conversion (TRS) than Trichoderma reesei, linked to higher β-glucosidase activity.
  • Hydrolysis yields exceeding 40% (TRS) were achieved with WM supplemented by a low dose of commercial enzyme.
  • Using WM eliminated the need for enzyme extraction/filtration, yielding similar results and reducing costs.
  • Combined WM and commercial cellulase allowed halving commercial enzyme dosage while increasing hydrolysis efficiency by up to 36%.

Conclusions:

  • Whole fermentation media from SSF is a cost-effective and efficient alternative for SESB enzymatic hydrolysis.
  • Eliminating enzyme extraction and filtration steps significantly reduces process costs and environmental impact.
  • This approach supports the economic viability and sustainability of biorefinery operations.