Antigen-specific lymphocyte transformation in congenital toxoplasmosis

Secreted (TSA) and water lysed antigens (WLA) derived from cell culture of the RH strain of Toxoplasma gondii have been used to induce antigen-specific mitogenesis of lymphocytes from children with congenital toxoplasmosis. Responsiveness to WLA was variable, with all patients giving false negative results. Responses to TSA, however, were highly specific, with no false negatives. This increased specificity was not due to an increased response to TSA (P less than 0.001), but a lower TSA response by the lymphocytes of the uninfected subjects (P greater than 0.1), compared with WLA in both cases. Preliminary studies have shown that while WLA induces high levels of non-specific immunity, such as natural killing, TSA is able to induce specific killing of Toxoplasma in vitro through the action of activated macrophages; this may partially explain the increased specificity of TSA in the lymphocyte transformation test.