Related Experiment Video
Updated: May 7, 2026
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
Moringa fruit inhibits LPS-induced NO/iNOS expression through suppressing the NF-κ B activation in RAW264.7 cells
Hyo-Jin Lee1, Yun-Jeong Jeong, Tae-Sung Lee
1Research Institute of Biomedical Engineering and Department of Medicine, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea.
Abstract:
In this study, we evaluated the anti-inflammatory effects of moringa (Moringa oleifera Lam.), a natural biologically active substance, by determining its inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophage RAW264.7 cells. Extracts from different parts of moringa (root, leaf, and fruit) reduced LPS-induced nitric oxide (NO) release in a dose-dependent manner. The moringa fruit extract most effectively inhibited LPS-induced NO production and levels of inducible nitric oxide synthase (iNOS). The moringa fruit extract also was shown to suppress the production of inflammatory cytokines including IL-1β, TNF-α, and IL-6. Furthermore, moringa fruit extract inhibited the cytoplasmic degradation of I κ B -α and the nuclear translocation of p65 proteins, resulting in lower levels of NF -κ B transactivation. Collectively, the results of this study demonstrate that moringa fruit extract reduces the levels of pro-inflammatory mediators including NO , IL-1β, TNF-α, and IL-6 via the inhibition of NF -κ B activation in RAW264.7 cells. These findings reveal, in part, the molecular basis underlying the anti-inflammatory properties of moringa fruit extract.
Insights
Moringa fruit extract effectively reduces inflammation by inhibiting key pro-inflammatory mediators like nitric oxide and cytokines. This natural substance works by suppressing the NF-κB signaling pathway in immune cells.
Area of Science:
- Pharmacology
- Immunology
- Natural Product Chemistry
Background:
- Inflammation is a complex biological response involving pro-inflammatory mediators.
- Macrophage activation by lipopolysaccharide (LPS) is a key model for studying inflammation.
- Moringa (Moringa oleifera Lam.) is recognized for its potential medicinal properties.
Purpose of the Study:
- To evaluate the anti-inflammatory effects of Moringa oleifera extracts.
- To determine the inhibitory mechanisms of moringa on pro-inflammatory mediators in LPS-stimulated macrophages.
- To identify which part of the moringa plant exhibits the most potent anti-inflammatory activity.
Main Methods:
- Utilized macrophage RAW264.7 cells stimulated with lipopolysaccharide (LPS).
- Assessed the inhibitory effects of moringa root, leaf, and fruit extracts on nitric oxide (NO) production.
- Measured levels of inducible nitric oxide synthase (iNOS), pro-inflammatory cytokines (IL-1β, TNF-α, IL-6), and NF-κB pathway components (IκB-α, p65).
Main Results:
- All moringa extracts reduced LPS-induced NO release in a dose-dependent manner.
- Moringa fruit extract demonstrated the strongest inhibition of NO production and iNOS levels.
- Moringa fruit extract suppressed IL-1β, TNF-α, and IL-6 production by inhibiting IκB-α degradation and p65 nuclear translocation, thereby reducing NF-κB activation.
Conclusions:
- Moringa fruit extract possesses significant anti-inflammatory properties.
- The anti-inflammatory effects are mediated through the inhibition of NF-κB signaling pathway activation.
- These findings elucidate the molecular mechanisms behind the anti-inflammatory potential of moringa fruit extract.
Related Concept Videos
02:26NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
02:26NF-kB-dependent Signaling Pathway