Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 7, 2026

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.4K

Genome editing using artificial site-specific nucleases in zebrafish.

Yu Hisano1, Satoshi Ota, Atsuo Kawahara

  • 1Laboratory for Cardiovascular Molecular Dynamics, RIKEN Quantitative Biology Center (QBiC), Furuedai 6-2-3, Suita, Osaka, 565-0874, Japan.

Development, Growth & Differentiation
|October 15, 2013
PubMed
Summary

Zebrafish genetics research benefits from new genome editing tools. Transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 enable precise gene modification in zebrafish, advancing organogenesis studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sinusoidal Obstruction Syndrome With Inotuzumab Ozogamicin Combined With Ponatinib for Philadelphia-Chromosome-Positive Lymphoblastic Malignancies.

EJHaem·2026
Same author

Impact of Culture Status on Outcomes in Vertebral Osteomyelitis: A Retrospective Cohort Study of 206 Patients.

Global spine journal·2026
Same author

Comprehensive understanding of the correlation between structure and harmonic properties of multicore superparamagnetic particles.

Nanoscale·2026
Same author

Migration of Fish Bones to the Bile Duct Following Hepaticojejunostomy.

DEN open·2026
Same author

Modified paravertebral foramen screw trajectory for posterior cervical spine fixation: feasibility of computed tomographic evaluation.

Asian spine journal·2026
Same author

Determinants of Initial Neurological Severity and Clinical Outcomes in Cervical Spine Dislocation Injuries.

Clinical spine surgery·2025

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Zebrafish are a key vertebrate model for genetic analysis, particularly in organogenesis.
  • Traditional forward genetics and reverse genetics (e.g., knockout mice) have limitations in vertebrates.
  • Establishing embryonic stem (ES) cells for gene targeting is challenging in most vertebrate models besides mice.

Purpose of the Study:

  • To review novel genome editing technologies for zebrafish research.
  • To highlight the application of TALEN and CRISPR/Cas9 in zebrafish gene targeting.

Main Methods:

  • Summarizing the principles of transcription activator-like effector nucleases (TALEN) technology.
  • Summarizing the principles of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system.
Keywords:
clustered regularly interspaced short palindromic repeats/Cas9genome editingheteroduplex mobility assaytranscription activator-like effector nucleases

More Related Videos

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.9K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K

Related Experiment Videos

Last Updated: May 7, 2026

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.4K
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.9K
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
05:55

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

1.7K
  • Discussing the application of these technologies for targeted genome modification in zebrafish.

    • Main Results:

    • TALEN and CRISPR/Cas9 systems allow direct and precise genome modification at targeted loci in zebrafish.
    • These technologies overcome limitations of previous gene targeting methods in zebrafish.
    • Facilitates the study of gene function through targeted gene disruption.

    Conclusions:

    • Genome editing technologies like TALEN and CRISPR/Cas9 are powerful tools for zebrafish research.
    • These methods significantly advance the study of organogenesis and gene function in zebrafish.
    • The application of these techniques broadens the scope of genetic analysis in this model organism.