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The RootScope: a simple high-throughput screening system for quantitating gene expression dynamics in plant roots.

Erin J Kast1, Minh-Duyen T Nguyen, Rosalie E Lawrence

  • 1Department of Biology, Swarthmore College, Swarthmore, PA, 19081, USA. nkaplin1@swarthmore.edu.

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|October 15, 2013
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Summary

Plants use complex heat shock responses for survival. The new RootScope system quantifies these responses in plant roots, revealing distinct mechanisms above and below 37°C.

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Stress Physiology

Background:

  • High temperature stress responses are critical for plant survival.
  • Plant high-temperature sensing mechanisms involve multiple pathways.
  • The RootScope system was developed to quantify heat shock responses in plant roots.

Purpose of the Study:

  • To develop an automated microscopy system for studying heat shock responses in plant roots.
  • To quantitate the dynamics and localization of heat shock factor (HSF) activation.
  • To investigate temperature-dependent differences in heat shock response.

Main Methods:

  • Development of the RootScope automated microscopy system.
  • Creation of a Hsp17.6p:GFP transcriptional reporter in Arabidopsis.
  • Quantitation of Hsp17.6p:GFP response dynamics using fluorescence microscopy.

Main Results:

  • Hsp17.6p:GFP expression increased with temperature from 28°C to 37°C.
  • Distinct kinetics and localization of the heat shock response were observed at 40°C compared to 37°C.
  • Hsp17.6p:GFP expression exhibited wave-like dynamics in growing roots.

Conclusions:

  • The RootScope system provides a powerful platform for investigating plant heat shock responses.
  • Different mechanisms may mediate heat shock responses below and above 37°C.
  • The study reveals dynamic and temperature-specific aspects of heat shock gene expression in plant roots.